Protocols

List of different protocols used in the lab.

Immunofluorescence

PCR from Worm Lysate

PCR from Genomic Prep

Live Imaging/Embryo Microscopy

coCRISPR RNP Mix Assembly

Bacterial Transformation (10Beta)

Feeding RNAi

Scoring Apoptosis

Freezing Worms

Bleaching Worms

Worm Viability Assay

Worm Viability Spreadsheet

Worm Genomic Prep

Trizol Extraction of RNA

Ethanol Precipitation

Co-immunoprecipitation

Worm Lysate Prep & Western Blot Analysis

HeLa Cell Immunostaining

10Beta Competent Cell Prep