PCR from Genomic Prep

PCR from genomic template: 

Initially, try a regular PCR protocol: PCR Program:
 * 1 ul genomic DNA
 * 1.5 ul 10uM each primer
 * 10 ul Phusion buffer
 * 5 ul 2mM dNTPs
 * dH2O to 49ul
 * add 1 ul Phusion
 * 94 ° 30 sec
 * 35 cycles:
 * 94 ° 30 sec
 * 5 ° below the melting temperature of your primers 30 sec
 * 72 ° 1 min (the standard is 30 sec-1 min/ kb of PCR product)
 * 72 ° 10 min
 * Hold at 15 °

If that doesn't work, try a Touchdown PCR:
 * 94 ° 30 sec
 * 10 cycles
 * 94 ° 30 sec
 * 1 ° degree above the melting temperature of your primers 10 cycles and subtract 0.5 ° with every cycle (total of 5 ° ) 30 sec
 * 72 ° 1 min (the standard is 30 sec – 1 min/ kb of PCR product)
 * 25 cycles
 * 98 ° 30 sec
 * 4 ° degrees below the melting temperature of your primers 30 sec 25 cycles
 * 72 ° 1 min (the standard is 30 sec – 1 min/ kb of PCR product)
 * 72 ° 10 min
 * Hold at 15