Bleaching Worms

Full Plate

 * Be sure you have lots of gravid adult hermaphrodites on the plate.
 * Wash worms off plate with M9 media. Transfer to Eppendorf or conical tube depending on volume.
 * Spin worms down 2000RPM for 2 minutes. Drain supernatant.
 * Make fresh bleach solution: Mix equal volumes of 3:2 bleach:water and 1M NaOH. This must be made fresh the day you bleach.
 * Add bleach to worms. Vortex.  Spin worms down and drain supernatant. Worms can be in bleach solution for maximum 4 minutes.
 * Wash worms two times with M9 media (add M9, vortex, spin down and drain supernatant).
 * Resuspend carcasses and eggs in some volume of M9 and pipet onto plate.  If planning on synchronizing worms, pipet onto plate with no food (worms will arrest as L1s).

Spot Method

 * Make fresh bleach solution: mix equal volumes of 3:2 bleach:water and 1M NaOH.
 * Pipet 25ul onto plate off the square of bacteria.
 * Pick 15-20 gravid adult hermaphrodites into drop.
 * Next day, pick L1s that have crawled out of adult carcasses into bacteria onto a new plate.