Towbin's Buffer

10X Towbin's Buffer

 * 1) 30.3 g Tris Base
 * 2) 144 g Glycine
 * 3) dd H 2 O to 1L

1X Towbin's Transfer Buffer
Optional: add 1 ml 20% SDS (0.02%). May help with transfer of higher molecular weight proteins.
 * 1) 100 ml 10X stock
 * 2) 200 ml Methanol (20%)
 * 3) dd H 2 O to 1L