Live Microscopy (C. elegans Embryos)

Protocol Deltavision settings:
 * 1) Prepare 2% agarose pad.
 * 2) Dissect embryos from 2-4 young adult worms (simply cut worms in center using 30G1/2 needles) into a 10uL drop of 1X Egg Buffer on 22x22mm coverslip.
 * 3) Mount on 2% agarose pad, securing cover slip with small drops of 2% agarose.
 * 4) Circle areas containing embryos on back of slide using sharpie.
 * 1) Switch to #5 dichroic filter (for GFP/mCherry tags)
 * 2) Move UV filer into position (back of microscope)
 * 3) Use 20X objective to find embryos, image with 100X objective
 * 4) Z-stacks: 2micron
 * 5) Timing: 20 sec intervals, total imaging 1 hour
 * 6) Exposures: 0.05 sec (GFP-Membrane), 0.1sec (mCherry-H2B)