LB Media


 * 1) To 800 ml distilled H2O add:
 * 2) * 5 g yeast extract
 * 3) * 10 g NaCl
 * 4) * 10 g Bacto-Tryptone
 * 5) Adjust pH to 7.5 with NaOH
 * 6) Adjust final volume to 1 L and mix.
 * 7) Divide into ten 100 ml aliquots and label bottles.
 * 8) Autoclave for sterilization.