S Basal


 * 1) To 400 ml distilled H2O add:
 * 2) * 5.85 g NaCl
 * 3) * 1 g K2HPO4
 * 4) * 6 g KH2PO4
 * 5) * 1 ml cholesterol (5 mg/ml in ethanol)
 * 6) Bring final volume to 1 L and re-mix
 * 7) Label and divide into 10 100 ml aliquots
 * 8) Autoclave for sterilization

For 2L S Basal

 * 1) To 800 ml distilled H2O add:
 * 2) * 11.7 g NaCl
 * 3) * 2 g K2HPO4
 * 4) * 12 g KH2PO4
 * 5) * 2 ml cholesterol (5 mg/ml in ethanol)
 * 6) Bring final volume to 1 L and re-mix
 * 7) Label and divide into 10 100 ml aliquots
 * 8) Autoclave for sterilization

Making Complete S Basal (S Medium)

 * 1) Follow steps for S Basal as directed above
 * 2) Let cool and to each bottle add aseptically:
 * 3) 1 ml 1M Potassium Citrate, pH 6.0
 * 4) 1 ml Trace Metals solution
 * 5) 300 μl 1M MgSO4
 * 6) 300 μl 1M CaCl2