LB Agar

Plates cannot stay indefinitely at room temperature so make sure you remind someone to bag the plates!
 * 1) Add to 1000 ml in a 2 L flask (agar will not go into solution):
 * 2) 10 g Bacto-tryptone
 * 3) 5 g Yeast extract
 * 4) 10 g NaCl
 * 5) 20 g Agar
 * 6) Sterilize by autoclaving. Keep stir bar in flask during autoclaving.
 * 7) After autoclaving, place flask on stir plate and stir slowly. Cool so that you can touch flask with bare hands (around 55°C). If necessary, add 1 ml antibiotic (if you add it while the media is still hot the antibiotic will not work) and mix. Pour into petri dishes.
 * 8) Mark petri dishes with marker to indicate what types of plates they are. See below.
 * 9) When agar has solidified, turn plates over to dry upside down for one day. Ask someone to bag them and put in the cold room.