50X Tris-Acetate for Electrophoresis (TAE)

50X Tris-Acetate for Electrophoresis (TAE)

 * 1) to 600 ml distilled H2O add:
 * 2) * 242 g Tris Base
 * 3) * 57.1 ml glacial Acetic Acid
 * 4) * 100 ml 0.5M EDTA (pH 8.0)
 * 5) Mix and completely dissolve
 * 6) Adjust final volume to 1L and remix
 * 7) Label and pour into 1L bottle
 * 8) Autoclave for sterilization

Making 2L of 50X TAE

 * 1) to 1200 ml distilled H2O add:
 * 2) * 484 g Tris Base
 * 3) * 114.2 ml glacial Acetic Acid
 * 4) * 200 ml 0.5M EDTA (pH 8.0)
 * 5) Mix and completely dissolve
 * 6) Adjust final volume to 1L and remix
 * 7) Label and pour into 1L bottle
 * 8) Autoclave for sterilization