Synthetic Complete Dropout Media

+ 100 g Adenine Sulfate
 * 1) 26.7 g Dropout Base (DOB)
 * 2) + appropriate amino acid supplement mixture
 * 3) * SC-Leu: 1.83 g/L
 * 4) * SC-Leu-Trp: 1.74 g/L
 * 5) * SC-Leu-Trp-His: 1.66 g/L
 * 1) Millipore water to 1 liter

For Plates:

 * 1) Add 20 grams agar
 * 2) Adjust the pH to 6.0 with HCl
 * 3) Autoclave at 121 °C for 25 minutes
 * 4) Cool to 55 °C and dispense into sterile petri dishes
 * 5) Store plates when solidified upside down at 4 °C

Plates Containing 3-Amino- 1,2,4- Triazole (3AT)

 * 1) Follow the previous recipe for SC-Leu-Trp-His
 * 2) Cool to approximately 65 °C
 * 3) Add 3AT as powder, stir to dissolve, then pour plates without further adjusting the pH
 * 4) Make plates with 10 mM, 25 mM, 75 mM, and 100 mM 3At to test auto-activation of DB fusion