Stocked Reagents

1 M CaCl2

 * 1) Dissolve 14.7 g per 100 mls distilled H2O
 * 2) Autoclave.

1 M MgCl2

 * 1) Dissolve 20.3 g per 100 mls distilled H2O
 * 2) Autoclave.

1 M NaCl

 * 1) Dissolve 5.8 g per 100 mls distilled H2O
 * 2) Autoclave.

1 M MgSO4

 * 1) Dissolve 12.0 g per 100 mls distilled H2O
 * 2) Autoclave

5 mg/ml Cholesterol in EtOH

 * 1) Dissolve 1 g cholesterol in 200 ml EtOH.
 * 2) Filter sterilize. DO NOT AUTOCLAVE!

10 N NaOH

 * 1) Dissolve 40 g per 100 mls distilled H2O
 * 2) Store in 50 ml conical tubes.
 * 3) DO NOT AUTOCLAVE!

10 N KOH

 * 1) dissolve 56.1 per 100 ml distilled H2O
 * 2) Store in 50 ml conical tubes.
 * 3) DO NOT AUTOCLAVE!