Directions for Autoclave

Autoclaving Dishes

 * Beakers and graduated cylinders are autoclaved uncovered.
 * Bottles are autoclaved with their caps on but not completely screwed on.
 * Rule of thumb: screw cap on tight and then loosen half a turn.
 * Flasks are capped with foil.


 * Put autoclave tape on the cap or foil of both bottles and flasks so any users know that they are sterile.
 * Autoclave time for dishes (choose dry run):
 * sterilization ~ 20 minutes
 * dry ~ 20 minutes
 * Put dishes back in their cabinet after autoclaving, once cooled.

Autoclaving Liquids or Fluids

 * Liquids in bottles are autoclaved with their caps on but not completely screwed on.
 * Liquids in flasks are autoclaves with a foil cap.
 * Put autoclave tape on the cap or foil of both bottles and flasks so any users know that they are sterile.
 * Autoclave time for fluids (choose fluid run):
 * sterilization ~40 minutes
 * Remember to reset jacket pressure after a fluid run!
 * Put bottles on the shelf after autoclaving.
 * Place flasks with media on a stir plate after autoclaving to let cool before adding antibiotic or pouring plates.