10X PBS


 * 1) To 800 ml distilled H2O add:
 * 2) * 80 g NaCl
 * 3) * 2.0 g KCl
 * 4) * 14.4 g Na2HPO4
 * 5) * 2.4 g KH2PO4
 * 6) Adjust pH to 7.4
 * 7) Adjust final volume to 1L and re-mix
 * 8) Label and pour into 1 L bottle
 * 9) Autoclave for sterilization

Making 1X PBST

 * 1) Make 10X PBS as directed above
 * 2) Once cool add to 10L Carboy
 * 3) Add 20 ml Tween
 * 4) Fill to final volume of 10L and mix well

Making High Salt 1X PBST

 * 1) Follow directions for 10X PBST except add an additional 146.1 g NaCl
 * 2) Once cool add to 10L Carboy
 * 3) Add 20 ml Tween
 * 4) Fill to final volume of 10L and mix well