Bacterial Transformation (10-Beta Cells)

These cells are designed for quick transformation and transformation efficiency actually drops if you increase the time of ice incubation steps.

1. Thaw aliquot of 10B cells on ice

2. Add plasmid (generally 1uL)

3. Incubate on ice 2 minutes.

4. Heatshock 42°C for 30 seconds.

5. Incubate on ice 1 minute.

6. Add 300uL SOC media (sterile technique) to cells.

7. Mix well and plate cells on LB + Carb plate.
 * NOTE: If you are using KAN or other resistance marker, you need to shake the cells in SOC for 30 minutes prior to plating, but this step is not necessary for carb/amp resistance plasmids.