Ethanol Precipitation of DNA

Reagents Needed

 * 3 M sodium acetate pH 5.2 or 5 M ammonium acetate
 * DNA
 * 100% ethanol

Protocol

 * 1) Measure the volume of the DNA sample.
 * 2) Add 1/10 volume of sodium acetate, pH 5.2, (final concentration of 0.3 M)
 * 3) * These amounts assume that the DNA is in TE only; if DNA is in a solution containing salt, adjust salt accordingly to achieve the correct final concentration.
 * 4) Mix well.
 * 5) Add 2 to 2.5 volumes of cold 100% ethanol (calculated after salt addition).
 * 6) Mix well.
 * 7) Place on ice or at -20 degrees C for >20 minutes
 * 8) Spin a maximum speed in a microfuge 10-15 min.
 * 9) Carefully decant supernatant.
 * 10) Add 1 ml 70% ethanol. Mix. Spin briefly. Carefully decant supernatant.
 * 11) Air dry or briefly vacuum dry pellet.
 * 12) Resuspend pellet in the appropriate volume of TE or water.